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A study of process interactions between cell disruption and debris clarification stages in the recovery of yeast intracellular products.

Clarkson, AI; Lefevre, P; Titchener-Hooker, NJ; (1993) A study of process interactions between cell disruption and debris clarification stages in the recovery of yeast intracellular products. Biotechnol Prog , 9 (5) pp. 462-467. 10.1021/bp00023a004.

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Abstract

The effects of varying the levels of cell disruption achieved by high-pressure homogenization upon subsequent centrifugal separation of cell debris have been defined by measuring sedimentation velocity distributions of the cell debris. Using this data together with particle size distributions, a computer-based model of a disk stack centrifuge has been used to predict the efficiency of centrifugal separation. These predictions have been compared with both laboratory scale and pilot plant scale experimental results. A similar sedimentation velocity technique has been used to characterize the properties of the flocs formed by addition of poly(ethylene imine) to a borax-clarified homogenate supernatant. The variations of floc settling properties with respect to polymer concentration are defined.

Type: Article
Title: A study of process interactions between cell disruption and debris clarification stages in the recovery of yeast intracellular products.
Location: United States
DOI: 10.1021/bp00023a004
Keywords: Biotechnology, Cell Separation, Centrifugation, Computer Simulation, Flocculation, Fungal Proteins, Intracellular Fluid, Saccharomyces cerevisiae
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Engineering Science Faculty Office
URI: http://discovery.ucl.ac.uk/id/eprint/1338755
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