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Enhancement of protein precipitate strength and density by low-frequency conditioning

Titchener-Hooker, NJ; McIntosh, RV; (1992) Enhancement of protein precipitate strength and density by low-frequency conditioning. Bioprocess Engineering , 8 (1-2) pp. 91-97. 10.1007/BF00369270.

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Abstract

The use of a continuous, low-frequency conditioning process to alter the structure of protein precipitate aggregates is examined. An increase in the density of aggregates is correlated with the levels of fluid acceleration and hence hydrodynamic stress to which the aggregates are exposed during conditioning. A combination of low-frequency conditioning followed by shear break-up (as in the feed zone to a high-speed disk-stack centrifuge) is shown to result in a precipitate suspension of increased particle size at the fine end of the distribution, and having a greater sedimentation velocity. The resistance of large aggregates to shear disruption is increased by low-frequency conditioning. © 1992 Springer-Verlag.

Type: Article
Title: Enhancement of protein precipitate strength and density by low-frequency conditioning
DOI: 10.1007/BF00369270
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Engineering Science Faculty Office
URI: http://discovery.ucl.ac.uk/id/eprint/1338752
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