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Physical and transcript map of the hereditary prostate cancer region at Xq27

Stephan, DA; Howell, GR; Teslovich, TM; Coffey, AJ; Smith, L; Bailey-Wilson, JE; Malechek, L; ... Trent, JM; + view all (2002) Physical and transcript map of the hereditary prostate cancer region at Xq27. GENOMICS , 79 (1) 41 - 50. 10.1006/geno.2001.6681.

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Abstract

We have recently mapped a locus for hereditary prostate cancer (termed HPCX) to the long arm of the X chromosome (Xq25-q27) through a genome-wide linkage study. Here we report the construction of an similar to 9-Mb sequence-ready bacterial clone contig map of Xq26.3-q27.3. The contig was constructed by screening BAC/PAC libraries with markers spaced at similar to 85-kb intervals. We identified overlapping clones by end-sequencing framework clones to generate 407 new sequence-tagged sites, followed by PCR verification of overlaps. Contig assembly was based on clone restriction fingerprinting and the landmark information. We identified a minimal overlap contig for genomic sequencing, which has yielded 7.7 Mb of finished sequence and 1.5 Mb of draft sequence. The transcriptional mapping effort localized 57 known and predicted genes by database searching, STS content mapping, and sequencing, followed by sequence annotation. These transcriptional units represent candidate genes for HPCX and multiple other hereditary diseases at Xq26.3-q27.3.

Type: Article
Title: Physical and transcript map of the hereditary prostate cancer region at Xq27
DOI: 10.1006/geno.2001.6681
Keywords: physical mapping, genome mapping, genome sequencing, contig mapping, bacterial artificial chromosomes, P1 artificial chromosomes, prostate cancer, Xq26.3-q27.3, sequence annotation, repetitive elements, ALBINISM-DEAFNESS SYNDROME, SUSCEPTIBILITY LOCUS, LINKAGE ANALYSIS, GENE, CHROMOSOME, GENOME, IDENTIFICATION, LOCALIZATION, ANOPHTHALMOS, SEARCH
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
URI: http://discovery.ucl.ac.uk/id/eprint/133601
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