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Mycobacterium tuberculosis H37Rv sigC is expressed from two promoters but is not auto-regulatory.

Chang, A; Smollett, KL; Gopaul, KK; Chan, BHY; Davis, EO; (2012) Mycobacterium tuberculosis H37Rv sigC is expressed from two promoters but is not auto-regulatory. Tuberculosis (Edinb) , 92 (1) pp. 48-55. 10.1016/j.tube.2011.09.005.

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Abstract

The extracytoplasmic function (ECF) sigma factor SigC has been implicated in the pathogenesis of Mycobacterium tuberculosis but control of its expression and activity is poorly understood. No proteins that interact with SigC have been detected leading to the suggestion that this sigma factor may be primarily controlled at the level of transcription. It has been suggested that SigC may be autoregulatory and a role has also been proposed for SigF in the expression of sigC. In this study we identified two promoters that were active under standard growth conditions by a combination of transcript start site mapping and promoter-lacZ fusion assays. The dominant promoter, P1, closely resembled mycobacterial SigA-dependent promoters, and introduction of a single base change at the conserved A of the -10 region eliminated promoter activity. Although the sequence of the other, P2, closely resembled the reported SigC consensus motifs, expression directed by this promoter was unaltered in a ΔsigC mutant strain, or in strains defective in other ECF sigma factors for which some similarity in consensus sequences was apparent. Comparison of the effects of different changes in the -10 region suggested that the P2 promoter was most likely recognised by SigA.

Type: Article
Title: Mycobacterium tuberculosis H37Rv sigC is expressed from two promoters but is not auto-regulatory.
Location: Scotland
DOI: 10.1016/j.tube.2011.09.005
Keywords: Animals, Bacterial Proteins, DNA-Binding Proteins, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Humans, Mycobacterium tuberculosis, Oligonucleotide Array Sequence Analysis, Promoter Regions, Genetic, Sigma Factor
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1329390
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