UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

The relationship between blood flow and neuronal activity in the rodent olfactory bulb

Chaigneau, E; Tiret, P; Lecoq, J; Ducros, M; Knopfel, T; Charpak, S; (2007) The relationship between blood flow and neuronal activity in the rodent olfactory bulb. J NEUROSCI , 27 (24) 6452 - 6460. 10.1523/JNEUROSCI.3141-06.2007.

Full text not available from this repository.

Abstract

In the brain, neuronal activation triggers an increase in cerebral blood flow ( CBF). Here, we use two animal models and several techniques ( two-photon imaging of CBF and neuronal calcium dynamics, intracellular and extracellular recordings, local pharmacology) to analyze the relationship between neuronal activity and local CBF during odor stimulation in the rodent olfactory bulb. Application of glutamate receptor antagonists or tetrodotoxin directly into single rat olfactory glomeruli blocked postsynaptic responses but did not affect the local odor-evoked CBF increases. This suggests that in our experimental conditions, odor always activates more than one glomerulus and that silencing one of a few clustered glomeruli does not affect the vascular response. To block synaptic transmission more widely, we then superfused glutamate antagonists over the surface of the olfactory bulb in transgenic G-CaMP2 mice. This was for two reasons: ( 1) mice have a thin olfactory nerve layer compared to rats and this will favor drug access to the glomerular layer, and ( 2) transgenic G-CaMP2 mice express the fluorescent calcium sensor protein G-CaMP2 in mitral cells. In G-CaMP2 mice, odor-evoked, odor-specific, and concentration-dependent calcium increases in glomeruli. Superfusion of glutamate receptor antagonists blocked odor-evoked postsynaptic calcium signals and CBF responses. We conclude that activation of postsynaptic glutamate receptors and rises in dendritic calcium are major steps for neurovascular coupling in olfactory bulb glomeruli.

Type: Article
Title: The relationship between blood flow and neuronal activity in the rodent olfactory bulb
DOI: 10.1523/JNEUROSCI.3141-06.2007
Keywords: two-photon microscopy, olfactory bulb, neurovascular coupling, field potential, mitral cell, blood flow, ACTIVITY-DEPENDENT INCREASES, SOURCE DENSITY ANALYSIS, RAT MITRAL CELLS, IN-VIVO, ODORANT REPRESENTATIONS, GLUTAMATE RECEPTORS, PYRAMIDAL NEURONS, DENDRITIC CALCIUM, FIELD POTENTIALS, IMAGING SIGNALS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/1329126
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item