UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis

Talbot, CB; McGinty, J; Grant, DM; McGhee, EJ; Owen, DM; Zhang, W; Bunney, TD; (2008) High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis. J BIOPHOTONICS , 1 (6) 514 - 521. 10.1002/jbio.200810054.

Full text not available from this repository.

Abstract

We report an automated optically sectioning fluorescence lifetime imaging (FLIM) multiwell plate reader for high content analysis (HCA) in drug discovery and accelerated research in cell biology. The system utilizes a Nipkow disc confocal microscope and performs, unsupervised FLIM, with autofocus, automatic setting of acquisition parameters and automated localisation of cells in the field of view. We demonstrate its applications to test dye solutions, fixed and live cells and FLIM-FRET.A fluorescence lifetime map showing automatically acquired data from a cell where the fluorophores are undergoing FRET. The acquisition time including stage movement, auto-find and auto-exposure was similar to 20 s.[GRAPHICS](c) 2008 by WILEY-VCH Velag GmbH & Co. KGaA. Weinheim

Type: Article
Title: High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis
DOI: 10.1002/jbio.200810054
Keywords: Fluorescence lifetime imaging, time-resolved imaging, high content analysis, unsupervised acquisition, CELL, MICROSCOPY
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of)
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1326653
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item