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Fusicoccin signaling reveals 14-3-3 protein function as a novel step in left-right patterning during amphibian embryogenesis

Bunney, TD; De Boer, AH; Levin, M; (2003) Fusicoccin signaling reveals 14-3-3 protein function as a novel step in left-right patterning during amphibian embryogenesis. DEVELOPMENT , 130 (20) 4847 - 4858. 10.1242/dev.00698.

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Abstract

To gain insight into the molecular mechanisms underlying the control of morphogenetic signals by H+ flux during embryogenesis, we tested Fusicoccin-A (FC), a compound produced by the fungus Fusicoccum amygdali Del. In plant cells, FC complexes with 14-3-3 proteins to activate H+ pumping across the plasma membrane. It has long been thought that FC acts on higher plants only; here, we show that exposing frog embryos to FC during early development specifically results in randomization of the asymmetry of the left-right (LR) axis (heterotaxia). Biochemical and molecular-genetic evidence is presented that 14-3-3-famfly proteins are an obligate component of Xenopus FC receptors and that perturbation of 14-3-3 protein function results in heterotaxia. The subcellular localization of 14-3-3 mRNAs and proteins reveals novel cytoplasmic destinations, and a left-right asymmetry at the first cell division. Using gain-of-function and loss-of-function experiments, we show that 14-3-3E protein is likely to be an endogenous and extremely early aspect of LR patterning. These data highlight a striking conservation of signaling pathways across kingdoms, suggest common mechanisms of polarity establishment between C. elegans and vertebrate embryos, and uncover a novel entry point into the pathway of left-right asymmetry determination.

Type:Article
Title:Fusicoccin signaling reveals 14-3-3 protein function as a novel step in left-right patterning during amphibian embryogenesis
DOI:10.1242/dev.00698
Keywords:left-right asymmetry, 14-3-3 protein, fusicoccin, Xenopus, LEFT-RIGHT ASYMMETRY, MEMBRANE H+-ATPASE, 1ST CLEAVAGE PLANE, DEVELOPING XENOPUS-EMBRYOS, JUNCTIONAL COMMUNICATION, SEPARATE MECHANISMS, INTRACELLULAR PH, NODAL EXPRESSION, SITUS-INVERSUS, AXIS FORMATION
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Structural and Molecular Biology

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