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Structural Insights into Formation of an Active Signaling Complex between Rac and Phospholipase C Gamma 2

Bunney, TD; Opaleye, O; Roe, SM; Vatter, P; Baxendale, RW; Walliser, C; Everett, KL; ... Katan, M; + view all (2009) Structural Insights into Formation of an Active Signaling Complex between Rac and Phospholipase C Gamma 2. MOL CELL , 34 (2) 223 - 233. 10.1016/j.molcel.2009.02.023.

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Abstract

Rho family GTPases are important cellular switches and control a number of physiological functions. Understanding the molecular basis of interaction of these GTPases with their effectors is crucial in understanding their functions in the cell. Here we present the crystal structure of the complex of Rac2 bound to the split pleckstrin homology (spPH) domain of phospholipase C-gamma(2) (PLC gamma(2)). Based on this structure, we illustrate distinct requirements for PLC gamma(2) activation by Rac and EGF and generate Rac effector mutants that specifically block activation of PLC gamma(2), but not the related PLC beta(2) isoform. Furthermore, in addition to the complex, we report the crystal structures of free spPH and Rac2 bound to GDP and GTP gamma S. These structures illustrate a mechanism of conformational switches that accompany formation of signaling active complexes and highlight the role of effector binding as a common feature of Rac and Cdc42 interactions with a variety of effectors.

Type: Article
Title: Structural Insights into Formation of an Active Signaling Complex between Rac and Phospholipase C Gamma 2
DOI: 10.1016/j.molcel.2009.02.023
Keywords: GTPASE-BINDING DOMAIN, RHO-GTPASES, CRYSTAL-STRUCTURE, EFFECTOR PROTEINS, FAMILY, STIMULATION, SWITCH, CDC42, TRANSDUCTION, C-GAMMA(2)
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1326641
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