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3D reconstruction of a micro pipette tip

Ostadi, H; Malboubi, M; Prewett, PD; Jiang, K; (2009) 3D reconstruction of a micro pipette tip. In: MICROELECTRONIC ENGINEERING. (pp. 868 - 870). ELSEVIER SCIENCE BV

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Abstract

Glass micro pipettes have been widely used for patch clamping. Although many researchers agree that the shape and roundness of pipette tip are important for cell grabbing and gigaseal formation, no numerical values of the geometry have been reported so far. In this research, a FIB/SEM system was used as a nano tomography tool to obtain the 3D shape of a pipette tip. The process involves a cycle of cutting a slice of the pipette using focused ion beam (FIB), taking an SEM image of the new surface, and then milling and imaging again to produce a stack of SEM images. A 3D reconstruction of the pipette was made based on the SEM images. The roundness of the pipette tip was measured and is presented in this paper for the first time. (C) 2008 Elsevier B.V. All rights reserved.

Type: Proceedings paper
Title: 3D reconstruction of a micro pipette tip
Event: 34th International Conference on Micro- and Nano-Engineering
Location: Athens, GREECE
Dates: 2008-09-15 - 2008-09-18
DOI: 10.1016/j.mee.2008.11.041
Keywords: Focused ion beam, Patch clamping, 3D reconstruction, Micro pipette, 3-DIMENSIONAL RECONSTRUCTION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: http://discovery.ucl.ac.uk/id/eprint/1325581
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