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Behavior of a cell line derived from normal human hepatocytes on nonphysiological and physiological-type substrates: Evidence for enhancement of secretion of liver-specific proteins by a three-dimensional growth pattern

Smalley, M; Leiper, K; Floyd, D; Mobberley, M; Ryder, T; Selden, C; ... Hodgson, H; + view all (1999) Behavior of a cell line derived from normal human hepatocytes on nonphysiological and physiological-type substrates: Evidence for enhancement of secretion of liver-specific proteins by a three-dimensional growth pattern. IN VITRO CELL DEV-AN , 35 (1) 22 - 32.

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Abstract

The behavior of a recently described cell line, HH25, derived fi om normal human hepatocytes, has been investigated on several different substrates-tissue-culture plastic, glass, a thin layer of rat-tail collagen I, and thin layers or thick gels of extracellular matrix derived from the Engelbreth-Holm-Swarm murine sarcoma (EHS matrix). Cellular morphology, proliferation, and secretion of three hepatocyte-specific proteins (albumin, alpha(1) acid glycoprotein, and alpha(1) antitrypsin) have been examined. There were no differences in morphology, proliferation, or differentiated function in the cells on either plastic, glass, collagen, I, or a thin layer of EHS matrix, but on a thick EHS matrix gel the cells altered their morphology (forming three-dimensional colonies with canalicular-like structures) and their production of albumin and alpha(1) acid glycoprotein was enhanced. This suggests that the enhanced differentiated function is associated with the morphological change (occurring only on the thick EHS gel) rather than with receptor-mediated cell-matrix interactions (which can also occur on the thin layer of EHS matrix). This cell line is therefore a good in vitro cellular model for the investigation of the roles of morphological changes and of cell-cell and cell-matrix interactions in the control of human hepatocyte behavior without the need for an extensive source of primary tissue.

Type:Article
Title:Behavior of a cell line derived from normal human hepatocytes on nonphysiological and physiological-type substrates: Evidence for enhancement of secretion of liver-specific proteins by a three-dimensional growth pattern
Keywords:EHS matrix, morphology, differentiation, albumin, canaliculi, EXTRACELLULAR-MATRIX COMPONENTS, MAMMARY GENE-EXPRESSION, RAT HEPATOCYTES, BASEMENT-MEMBRANE, SANDWICH CONFIGURATION, FUNCTIONAL-DIFFERENTIATION, PRIMARY CULTURE, INDUCTION, INVITRO, MODULATION

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