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Comparison of three solid phase supports for promoting three-dimensional growth and function of human liver cell lines

Selden, C; Roberts, E; Stamp, G; Parker, K; Winlove, P; Ryder, T; ... Hodgson, H; + view all (1998) Comparison of three solid phase supports for promoting three-dimensional growth and function of human liver cell lines. ARTIF ORGANS , 22 (4) 308 - 319.

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Abstract

An extracorporeal liver support system will require that liver cells maintain their normal differentiated function, This is more likely to be achieved utilizing a three-dimensional culture configuration rather than a simple monolayer culture. We present data on a human liver cell line attached and maintained on different three-dimensional supports, porous glass (Siran), silicon (Immobasil), and calcium-alginate beads. Albumin, fibrinogen, prothrombin, alpha 1-acid glycoprotein and alpha(1)-antitrypsin secretions were measured, Proliferation was slower on each of the three-dimensional supports than on the monolayer culture, The protein secretion of all 5 proteins was highest in cells encapsulated in alginate, silicon beads supported greater protein secretion than glass. Cells on silicon or within alginate were rounded; those on glass grew in 2 configurations as flattened monolayers and as rounded colonies. Cells in alginate secreted as much protein as the whole liver (e.g., albumin, 14.88 g/10(12) cells/day compared to the whole liver, similar to 12 g/day). Three-dimensional culture of a human liver cell line leads to both proliferation and a high synthetic capacity? an important feature of cells suitable for an extracorporeal liver support system.

Type:Article
Title:Comparison of three solid phase supports for promoting three-dimensional growth and function of human liver cell lines
Keywords:hepatocyte culture, three-dimensional supports, protein secretion, LONG-TERM CULTURE, EXTRACELLULAR-MATRIX GEOMETRY, PRIMARY RAT HEPATOCYTES, SANDWICH CONFIGURATION, TRANSCRIPTION FACTORS, DIFFERENTIATION, MAINTENANCE, SHEAR, PROLIFERATION, SPHEROIDS

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