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Alginate-encapsulated HepG2 Cells in a Fluidized Bed Bioreactor Maintain Function in Human Liver Failure Plasma

Coward, SM; Legallais, C; David, B; Thomas, M; Foo, Y; Mavri-Damelin, D; ... Selden, C; + view all (2009) Alginate-encapsulated HepG2 Cells in a Fluidized Bed Bioreactor Maintain Function in Human Liver Failure Plasma. ARTIF ORGANS , 33 (12) 1117 - 1126. 10.1111/j.1525-1594.2009.00821.x.

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Abstract

Alginate-encapsulated HepG2 cells cultured in microgravity have the potential to serve as the cellular component of a bioartificial liver. This study investigates their performance in normal and liver failure (LF) human plasma over 6-8 h in a fluidized bed bioreactor. After 8 days of microgravity culture, beads containing 1.5 x 109 cells were perfused for up to 8 h at 48 mL/min with 300 mL of plasma. After exposure to 90% LF plasma, vital dye staining showed maintained cell viability, while a 7% increase in lactate dehydrogenase activity indicated minimal cell damage. Glucose consumption, lactate production, and a 4.3-fold linear increase in alpha-fetoprotein levels were observed. Detoxificatory function was demonstrated by quantification of bilirubin conjugation, urea synthesis, and Cyp450 1A activity. These data show that in LF plasma, alginate-encapsulated HepG2 cells can maintain viability, and metabolic, synthetic, and detoxificatory activities, indicating that the system can be scaled-up to form the biological component of a bioartificial liver.

Type:Article
Title:Alginate-encapsulated HepG2 Cells in a Fluidized Bed Bioreactor Maintain Function in Human Liver Failure Plasma
DOI:10.1111/j.1525-1594.2009.00821.x
Keywords:Artificial liver, Bioreactors, Tumor cell line, Hepatocytes, Plasma, Liver failure, FULMINANT HEPATIC-FAILURE, HEPATOCYTE GROWTH-FACTOR, BIOARTIFICIAL LIVER, RAT HEPATOCYTES, CONTROLLED-TRIAL, SUPPORT-SYSTEMS, DNA-SYNTHESIS, SERUM, CULTURE

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