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Red cell PMVs, plasma membrane-derived vesicles calling out for standards

Hind, E; Heugh, S; Ansa-Addo, EA; Antwi-Baffour, S; Lange, S; Inal, J; (2010) Red cell PMVs, plasma membrane-derived vesicles calling out for standards. BIOCHEM BIOPH RES CO , 399 (4) 465 - 469. 10.1016/j.bbrc.2010.07.095.

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Abstract

Plasma membrane-derived vesicles (PMVs) or microparticles are vesicles (0.1-1 mu m in diameter) released from the plasma membrane of all blood cell types under a variety of biochemical and pathological conditions. PMVs contain cytoskeletal elements and some surface markers from the parent cell but lack a nucleus and are unable to synthesise macromolecules. They are also defined on the basis that in most cases PMVs express varying amounts of the cytosolic leaflet lipid phosphatidylserine, which is externalised during activation on their surface. This marks the PMV as a biologically distinct entity from that of its parent cell, despite containing surface markers from the original cell, and also explains its role in events such as phagocytosis and thrombosis. There is currently a large amount of variation between investigators with regard to the pre-analytical steps employed in isolating red cell PMVs or RPMVs (which are slightly smaller than most PMVs), with key differences being centrifugation and sample storage conditions, which often leads to result variability. Unfortunately, standardization of preparation and detection methods has not yet been achieved. This review highlights and critically discusses the variables contributing to differences in results obtained by investigators, bringing to light numerous studies of which RPMVs have been analysed but have not yet been the subject of a review. (C) 2010 Elsevier Inc. All rights reserved.

Type: Article
Title: Red cell PMVs, plasma membrane-derived vesicles calling out for standards
DOI: 10.1016/j.bbrc.2010.07.095
Keywords: Red cell, Plasma membrane-derived vesicles, Standardization, HUMAN-ERYTHROCYTES, CIRCULATING MICROPARTICLES, SCOTT-SYNDROME, BLOOD-CELLS, VESICULATION, RELEASE, MICROVESICLES, QUANTITATION, TRANSFUSION, HEMOSTASIS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/1323734
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