Graves, FM; Tinker, A; (2000) Functional expression of the pore forming subunit of the ATP-sensitive potassium channel in Saccharomyces cerevisiae. BIOCHEM BIOPH RES CO , 272 (2) 403 - 409.
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We have expressed the pore-forming subunits (Kir 6.1 and Kir 6.2) of the mammalian ATP-sensitive potassium channel in a potassium-transport deficient yeast strain (trk1 trk2). Functional expression of Kir 6.2 and Kir 6.1 can, complement growth deficiency weakly and strongly respectively of the yeast strain on low-potassium medium. Mutations of Kir 6.2 that abolish ATP sensitivity (K185Q, I182Q) and enhance trafficking to the plasma membrane surface (Kir 6.2 Delta C36) lead to significantly better growth rescue. Growth rescue of Kir 6.1, Kir 6.2 and the above mutants can be inhibited by pharmacological agents (cesium ions, phentolamine and quinine) known to decrease channel activity by direct interaction with the pore forming subunit. Thus we have developed a system in yeast that can report both loss and gain of function mutations in these subunits and pharmacological interventions. (C) 2000 Academic Press.
|Title:||Functional expression of the pore forming subunit of the ATP-sensitive potassium channel in Saccharomyces cerevisiae|
|Keywords:||Saccharomyces cerevisiae, ATP-sensitive potassium channel, inward rectifier, EMBRYONIC KIDNEY-CELLS, RECTIFYING K+ CHANNEL, PANCREATIC BETA-CELLS, SULFONYLUREA RECEPTOR, SKELETAL-MUSCLE, YEAST, DIAZOXIDE, KIR6.2, TRANSFORMATION, DETERMINANTS|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)|
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