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Prostaglandin D-2 inhibits C2C12 myogenesis

Velica, P; Khanim, FL; Bunce, CM; (2010) Prostaglandin D-2 inhibits C2C12 myogenesis. MOL CELL ENDOCRINOL , 319 (1-2) 71 - 78. 10.1016/j.mce.2010.01.023.

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Abstract

Muscle repair following injury is preceded by a rapid inflammatory response with myoblasts being exposed to high levels of prostaglandin D-2 (PGD(2)) from invading leukocytes We demonstrate that PGD(2) strongly inhibits C2C12 myogenesis as measured by cell fusion, creatine kinase activity and MyoD, myogenin and alpha-actin expression Inhibition of myogenesis required micromolar PGD(2) concentrations and was independent of the known PGD(2) receptors DP1 and DP2 Unlike its cyclopentenone derivative 15-deoxy-Delta(12.14)-PGJ(2) (15d-PGJ(2)). PGD(2) did not generate toxic mitochondrial superoxide Indicating that the inhibition of myogenesis is not mediated by generation of high concentrations of PGD(2)-derived 15d-PGJ(2) Thus our observations provide evidence for a novel PGD(2) signalling mechanism during muscle repair exclusively mediated by high inflammatory associated PGD(2) concentrations These findings Indicate a complex interplay between myoblasts and Inflammatory cells during the repair process and have implications for the use of non-steroidal anti-inflammatory drugs in the treatment of muscle injuries (C) 2010 Elsevier Ireland Ltd. All rights reserved.

Type: Article
Title: Prostaglandin D-2 inhibits C2C12 myogenesis
DOI: 10.1016/j.mce.2010.01.023
Keywords: Prostaglandin, Myogenesis, COX-2, NSAID, Healing, MUSCLE-CELL-DIFFERENTIATION, ACTIVATED RECEPTOR-GAMMA, SKELETAL-MUSCLE, MYOBLAST PROLIFERATION, PROGENITOR CELLS, COX-2 PATHWAY, PPAR-GAMMA, INFLAMMATION, REGENERATION, EXPRESSION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: http://discovery.ucl.ac.uk/id/eprint/1319384
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