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Expression of GTP-binding protein alpha subunits in human thymocytes

Kabouridis, PS; Waters, ST; Escobar, S; Stanners, J; Tsoukas, CD; (1995) Expression of GTP-binding protein alpha subunits in human thymocytes. Molecular and Cellular Biochemistry , 144 (1) 45 - 51. 10.1007/BF00926739.

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In this report, we investigate G protein α subunit diversity in human thymocytes, utilizing common properties shared by these genes and reverse transcription-polymerase chain reaction (RT-PCR). Sequence analysis of PCR amplified gene portions, indicate the presence of members from all four G- protein families that have been described thus far. The α subunit genes identified are: Gα(il-3) and Gα(z) but not Gα(o) from the G(i) family, Gα(s) from the G(s) family, Gα(11), Gα(q), and Gα(16) from the G(q) family, and Gα(12) and Gα(13) from the G(12) family. Also in this report we present the nucleotide and predicted amino acid sequences of the human Gα(13) cloned from a thymocyte cDNA library. The sequence of the human Gα(13) has not been previously reported. Comparison of this sequence with the reported murine Gα(13) shows >90% identity at the deduced amino acid sequence level. We conclude that thymocytes represent a useful experimental system for the study of G protein involvement in immune responses and lymphocyte development.

Title:Expression of GTP-binding protein alpha subunits in human thymocytes
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)

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