UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

A limited upstream region of the human sucrase-isomaltase gene confers glucose-regulated expression on a heterologous gene.

Rodolosse, A; Chantret, I; Lacasa, M; Chevalier, G; Zweibaum, A; Swallow, D; Rousset, M; (1996) A limited upstream region of the human sucrase-isomaltase gene confers glucose-regulated expression on a heterologous gene. Biochem J , 315 ( pp. 301-306.

Full text not available from this repository.

Abstract

We have previously shown that glucose can exert a repressive effect on the transcription of the sucrase-isomaltase (SI) gene in the differentiated enterocyte-like human colon carcinoma cell lines HT-29 and Caco-2. To characterize the region through which glucose exerts this effect, three different-length fragments of the 5'-flanking region of the human SI gene were linked to the reporter gene luciferase in an episomal vector carrying a hygromycin resistance gene. These fragments were used for transfection into a clone of the Caco-2 cell line, PF11, which has high glucose consumption and only expresses SI at high levels when cultured in the presence of a low supply of glucose. By using the stably transformed PF11 cells grown either in standard high glucose (25 mM) or in low glucose (1 mM) it was possible to show that the smallest fragment of the SI promoter, extending from bases -370 to +30, contains all the information required for the glucose repression of the reporter gene luciferase.

Type: Article
Title: A limited upstream region of the human sucrase-isomaltase gene confers glucose-regulated expression on a heterologous gene.
Location: England
Keywords: Base Sequence, Caco-2 Cells, DNA, Gene Expression Regulation, Neoplastic, Genes, Reporter, Glucose, Humans, Luciferases, Molecular Sequence Data, Promoter Regions, Genetic, Regulatory Sequences, Nucleic Acid, Sucrase-Isomaltase Complex
URI: http://discovery.ucl.ac.uk/id/eprint/1314973
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item