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Generation of transgenic plants expressing plasma membrane-bound antibodies to the environmental pollutant microcystin-LR

Barbi, T; Drake, PMW; Drever, M; van Dolleweerd, CJ; Porter, AR; Ma, JKC; (2011) Generation of transgenic plants expressing plasma membrane-bound antibodies to the environmental pollutant microcystin-LR. TRANSGENIC RES , 20 (3) 701 - 707. 10.1007/s11248-010-9431-4.

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Abstract

In this paper we describe the engineering and regeneration of transgenic tobacco plants expressing a recombinant plasma membrane-retained antibody specific to microcystin-LR (MC-LR), the environmental toxin pollutant produced by cyanobacteria. The antibody was created by a genetic fusion of the antigen binding regions of the microcystin-specific single chain antibody, 3A8, with the constant regions from the murine IgG1 kappa, Guy's 13, including a membrane retention sequence at the C-terminal end of the antibody heavy chain. The antibody produced in the leaves was shown to be functional by binding to MC-LR in an ELISA with antibody yields in transgenic plant leaves reaching a maximum of 1.2 mu g g(-1) leaf f.wt (0.005% total soluble protein). Antibody-MC-LR complexes formed in leaves after addition of MC-LR to hydroponic medium around the roots of transgenic plant cultures.

Type: Article
Title: Generation of transgenic plants expressing plasma membrane-bound antibodies to the environmental pollutant microcystin-LR
DOI: 10.1007/s11248-010-9431-4
Keywords: Microcystin-LR, Transgenic plants, Antibodies, Phytoremediation, MONOCLONAL-ANTIBODIES, TOBACCO PLANTS, PROTEIN, GROWTH, PHYTOREMEDIATION, TRANSFORMATION, GENES
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1314654
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