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Expression and plasma membrane localization of the mammalian B-cell receptor complex in transgenic Nicotiana tabacum

Barbi, T; Irons, SL; Pepponi, I; Hawes, C; Ma, JKC; Drake, PMW; (2011) Expression and plasma membrane localization of the mammalian B-cell receptor complex in transgenic Nicotiana tabacum. PLANT BIOTECHNOL J , 9 (4) 455 - 465. 10.1111/j.1467-7652.2010.00566.x.

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Abstract

P>The B-cell antigen receptor (BCR), displayed on the plasma membrane of mature B cells of the mammalian immune system, is a multimeric complex consisting of a membrane-bound immunoglobulin (mIg) noncovalently associated with the Ig alpha/Ig beta heterodimer. In this study, we engineered transgenic tobacco plants expressing all four chains of the BCR. ELISA, Western blotting and confocal microscopy demonstrated that the BCR was correctly assembled in plants, predominantly in the plasma membrane, and that the noncovalent link was detergent sensitive. This is the first example of a noncovalently assembled plasma membrane-retained heterologous receptor in plants. In B cells of the mammalian immune system, following antigen binding to mIg, BCR is internalized and tyrosine residues on Ig alpha and Ig beta are phosphorylated activating a signaling cascade through interaction with protein kinases that ultimately leads to the initiation of gene expression. Expression of the BCR may therefore be an important tool for the study of plant endocytosis and the identification of previously unknown plant tyrosine kinases. The specificity and diversity of the antibody repertoire, coupled to the signal transduction capability of the Ig alpha/Ig beta heterodimer, also indicates that plants expressing BCR may in future be developed as environmental biosensors.

Type: Article
Title: Expression and plasma membrane localization of the mammalian B-cell receptor complex in transgenic Nicotiana tabacum
DOI: 10.1111/j.1467-7652.2010.00566.x
Keywords: B-cell receptor, transgenic plants, antibody, RESONANCE ENERGY-TRANSFER, ANTIGEN RECEPTOR, RECONSTITUTION SYSTEM, LIVING CELLS, PLANTS, ANTIBODIES, IDENTIFICATION, GENERATION, INITIATION, VERSATILE
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Biosciences (Division of) > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1314650
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