Samsonova, JV and Fedorova, MD and Andreeva, IP and Rubtsova, MY and Egorov, AM (2010) Characterization of Anti-Chloramphenicol Antibodies by Enzyme-Linked Immunosorbent Assay. ANAL LETT , 43 (1) 133 - 141. 10.1080/00032710903276570.
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Polyclonal antibodies against conjugates of chloramphenicol succinate and chloramphenicol base with proteins were obtained and characterized in direct ELISA. Antiserum against a conjugate of chloramphenicol (CAP) base with BSA (direct coupling) was very specific and showed cross-reactivity only with CAP succinate (11.3%) and CAP base (4.6%); whereas, antisera against a conjugate of CAP succinate with a protein recognized CAP succinate strongly as an initial compound. In direct ELISA, antisera against a conjugate of CAP succinate with KLH (homologous assay) and CAP base with BSA (heterologous assay) showed similar sensitivity: IC50 were 1.3 and 1.5ngmL-1, respectively. Applicability of the immunoreagents obtained was shown in the analysis of CAP residues in milk (3.5% fat content). Detection limit of 0.3ngmL-1 was obtained for milk diluted 5 times.
|Title:||Characterization of Anti-Chloramphenicol Antibodies by Enzyme-Linked Immunosorbent Assay|
|Keywords:||Antibodies, Chloramphenicol, Enzyme-linked immunosorbent assay, PLASMON RESONANCE BIOSENSOR, MONOCLONAL-ANTIBODY, CHICKEN MUSCLE, RESIDUES, ELISA, MILK, IMMUNOASSAY, TISSUE, SHRIMP, HONEY|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Life Sciences > Laboratory for Molecular Cell Biology|
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