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Expression of P2X(6) receptors in the enteric nervous system of the rat gastrointestinal tract.
HISTOCHEM CELL BIOL
177 - 188.
Expression of P2X(4) and P2X(6) receptor subunits in the gastrointestinal tract of the rat was studied with double-labeling fluorescence immunohistochemistry. The results showed that P2X(6) receptors were expressed widely in the submucosal and myenteric plexuses. In the myenteric plexus, P2X(6) receptors were expressed mainly in large size neurons which resembled Dogiel type II neurons. These P2X(6) receptor-immunoreactive (ir) neurons also expressed calbindin 28K, calretinin and neuronal nuclei (NeuN), proteins that are markers of intrinsic sensory neurons. In the submucosal plexus, all the calbindin 28K, calretinin and NeuN-ir cells were immunoreactive for P2X(6) receptors. P2X(6) receptors do not form homomultimers, but rather heteromultimers with either P2X(2) or P2X(4) receptors. P2X(4) receptors were not expressed in neurons, but were expressed in macrophages of the rat gastrointestinal tract. These data indicate that P2X(6) receptors are mainly expressed on intrinsic sensory neurons and that ATP, via P2X(6) receptors probably in heteromeric combination with P2X(2) receptors, may be involved in regulating the physiological functions of these neurons.
|Title:||Expression of P2X(6) receptors in the enteric nervous system of the rat gastrointestinal tract|
|Keywords:||P2X(6) receptor, Enteric neuron, Gastrointestinal tract, Rat, PIG SMALL-INTESTINE, GUINEA-PIG, MYENTERIC NEURONS, CHOLINE-ACETYLTRANSFERASE, DISTAL COLON, IMMUNOREACTIVITY, MOUSE, IDENTIFICATION, PURINE, PLEXUS|
|UCL classification:||UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
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