Estrogen altered visceromotor reflex and P2X(3) mRNA expression in a rat model of colitis.
956 - 962.
P2X(3) and P2X(2/3) receptors are expressed in peripheral tissues and dorsal root ganglia (DRG) and participate in peripheral pain. However, the mechanisms underlying P2X receptor-mediated nociception at different ovarial hormone levels has not been examined. In this study, 24 female rats were randomly divided into sham-operated (sham), ovariectomized (OVX). estrogen-treated, and estrogen-progesterone-treated groups with colitis. In each group, the visceromotor reflex (VMR) to colorectal distension was tested and the DRG were harvested for a real-time PCR analysis of P2X(3) and P2X(2) receptor m RNA. In OVX rats with colitis we found that the VMR to colorectal distension and P2X(3) receptor mRNA in DRG were both significantly decreased. Estrogen replacement reversed the decrease. However, neither the VMR nor the P2X(3) mRNA level in DRG from OVX colitis rats was reversed by the complex of estrogen and progesterone. Patch-clamp recording showed that in colitis rats. estradiol rapidly potentiated the sustained and transient currents evoked by ATP to 336 +/- 49% and 122 +/- 12% of controls, respectively, in a subpopulation of DRG neurons, which were blocked by ICI 182, 780, an antagonist of the estrogen receptor. Whereas progesterone rapidly inhibited the transient currents induced by ATP to 67 +/- 10% of control and had no effect on the sustained currents evoked by the same agonist. These results indicate that P2X(3) receptors are likely to be an important contributor to the altered colonic functions in colitis rats, where the underlying mechanisms are closely related to endogenous estrogen modulation. (C) 2009 Elsevier Inc. All rights reserved.
|Title:||Estrogen altered visceromotor reflex and P2X(3) mRNA expression in a rat model of colitis|
|Keywords:||ATP, Dorsal root ganglion, Colitis, P2X(3), Ovariectomy, IRRITABLE-BOWEL-SYNDROME, ROOT GANGLION NEURONS, SENSORY NEURONS, MECHANOSENSORY TRANSDUCTION, COLORECTAL DISTENSION, ULCERATIVE-COLITIS, RECEPTOR-ALPHA, VISCERAL PAIN, DISEASE, HYPERSENSITIVITY|
|UCL classification:||UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
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