Kalea, AZ; Harris, PD; Klimis-Zacas, DJ; (2005) Dietary manganese suppresses alpha(1) adrenergic receptor-mediated vascular contraction. J NUTR BIOCHEM , 16 (1) 44 - 49. 10.1016/j.jnutbio.2004.09.002.
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We examined the effect of dietary manganese (Mn) on the vascular contractile machinery in rat thoracic aortas. Weanling male SpragueDawley rats were fed either an Mn-deficient (MnD), Mn-adequate (MnA) or Mn-supplemented (MnS) diet (< 1, 10-15 and 45-50 ppm Mn, respectively). After 15 weeks on the diets the rats were sacrificed and 3-min aortic rings were contracted in six cumulative doses of the alpha(1), adrenergic receptor agonist L-phenylephrine (L-Phe, 10(-8) to 3 X 10(-6) M) under 1.5-g preload and relaxed with one dose of acetylcholine (3 x 10(-6) M) to assess intact endothelium. The maximal force (F-max) of contraction and relaxation, as well as the vessel sensitivity (pD(2)) were determined. Manganese deficiency, assessed by hepatic Mn content, significantly lowered the rate of animal growth. A two-way analysis of variance revealed that MnS animals developed lower F-max when contracted with L-Phe compared with the MnD and MnA animals (Pless than or equal to001). Thus, dietary Mn at levels of 45-50 ppm affects the contractile machinery by reducing maximal vessel contraction to an alpha(1) adrenergic agonist. The observed pD(2) was significantly greater in the MnD group compared with the MnA and MnS animals (Pless than or equal to.001). Thus, restriction of dietary Mn affects vascular sensitivity to the alpha(1) adrenergic receptor. Our results demonstrate for the first time that dietary Mn influences the receptor signaling pathways and contractile machinery of vascular smooth muscle cells in response to an a, adrenergic receptor. (C) 2005 Elsevier Inc. All rights reserved.
|Title:||Dietary manganese suppresses alpha(1) adrenergic receptor-mediated vascular contraction|
|Keywords:||manganese, endothelium, vascular smooth muscle, vasoconstriction, alpha(1) adrenergic agonist, SMOOTH-MUSCLE-CELLS, SPRAGUE-DAWLEY RATS, ENDOTHELIAL DYSFUNCTION, NITRIC-OXIDE, MICROMOLAR CONCENTRATIONS, BLOOD-VESSELS, AORTA, ATHEROSCLEROSIS, HYPERTENSION, DEFICIENCY|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science|
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