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Inflammatory stress in primary venous and aortic endothelial cells of type 1 diabetic mice

Bucciarelli, LG; Pollreisz, A; Kebschull, M; Ganda, A; Kalea, AZ; Hudson, BI; ... Yan, SF; + view all (2009) Inflammatory stress in primary venous and aortic endothelial cells of type 1 diabetic mice. DIABETES VASC DIS RE , 6 (4) 249 - 261. 10.1177/1479164109338775.

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Abstract

Objective: The progression of diabetes is associated with profound endothelial dysfunction. We tested the hypothesis that cellular stress would be detectable in ECs retrieved from arterial and venous vessels of diabetic mice.Method: We describe a method for direct isolation of well-characterised aortic and venous ECs from mice in which cells are not subjected to propagation in culture.Results: Gene expression profiling, confirmed by real-time PCR, revealed a progressive increase in markers of injury within two main gene families, EC activation and EC apoptosis, in aortic and venous ECs recovered from diabetic versus non-diabetic mice. In short-term diabetes, IIIb mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. In long-term diabetes, casp-I mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls.Conclusion: These data suggest that diabetes imparts diffuse endothelial perturbation in the arterial and venous endothelium.

Type:Article
Title:Inflammatory stress in primary venous and aortic endothelial cells of type 1 diabetic mice
DOI:10.1177/1479164109338775
Keywords:vein, artery, endothelium, inflammation, diabetes, OXIDATIVE STRESS, VASCULAR ENDOTHELIUM, HEART-FAILURE, MELLITUS, ACTIVATION, APOPTOSIS, INHIBITION, MYOCARDIUM, PROTEASE, HUMANS
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science

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