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Role of neutrophil depletion and elastase inhibition in modifying skeletal muscle reperfusion injury.

Crinnion, JN; Homer-Vanniasinkam, S; Hatton, R; Parkin, SM; Gough, MJ; (1994) Role of neutrophil depletion and elastase inhibition in modifying skeletal muscle reperfusion injury. Cardiovasc Surg , 2 (6) 749 - 753.

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Abstract

This study investigated the effect of neutrophil depletion and neutrophil elastase inhibition on the severity of skeletal muscle reperfusion injury. In a rodent model, indices (experimental/normal limb) of gastrocnemius muscle viability (histochemical staining), oedema (wet:dry weight ratio) and myeloperoxidase content (neutrophil recruitment) were assessed in normal (no ischaemia), ischaemic (6-h unilateral hindlimb ischaemia), control (6-h ischaemia and 4-h reperfusion), neutrophil-depleted rats (given antineutrophil serum) and rats receiving the neutrophil elastase inhibitor Elafin. Neutrophil recruitment muscle infarction and oedema did not occur in normal limbs, or in those subjected to ischaemia without reperfusion. In contrast increased muscle myeloperoxidase levels (P < 0.001), muscle infarction (P < 0.01) and oedema (P < 0.001) all occurred in the reperfused limbs of control animals compared with those of normal and ischaemic rats. Antineutrophil serum and Elafin both reduced neutrophil recruitment during reperfusion (P < 0.001 and P < 0.01 respectively) and muscle viability was preserved. Reperfusion oedema still occurred however, suggesting that altered endothelial permeability is mediated by factors other than neutrophils.

Type:Article
Title:Role of neutrophil depletion and elastase inhibition in modifying skeletal muscle reperfusion injury.
Location:ENGLAND
Language:English
Keywords:Acute Disease, Animals, Disease Models, Animal, Edema, Hindlimb, Immune Sera, Ischemia, Leukocyte Elastase, Male, Muscle, Skeletal, Neutropenia, Neutrophils, Pancreatic Elastase, Peroxidase, Proteinase Inhibitory Proteins, Secretory, Proteins, Rats, Rats, Sprague-Dawley, Reperfusion Injury, Serine Proteinase Inhibitors, Time Factors
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of)

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