Do prostaglandins have a salutary role in skeletal muscle ischaemia-reperfusion injury?
EUR J VASC ENDOVASC
439 - 444.
Objectives: the effects of prostaglandins (PG) E-1,, E-2,, and the prostacyclin analogue iloprost with and without the addition of free-radical scavengers catalase and superoxide dismutase on gastrocnemius blood flow and oedema were studied in a rodent model of hindlimb ischaemia-reperfusion.Methods: male Sprague-Dawley rats underwent 6-h hindlimb ischaemia with 4-h reperfusion. Prostaglandins were infused prior to reperfusion and their effects on limb blood flow and oedema examined.Results: control animals exhibited a triphasic pattern of muscle blood flow during reperfusion compared to normal animals. PGE, did not abolish low reflow at 10 min, relative reperfusion was preserved but reperfusion injury was abolished at 120 min. Muscle blood flow was increased at 240 min compared to controls. Increased limb swelling was also seen. Addition of free-radical scavengers caused the abolition of low reflow. Similar results were seen with iloprost. PGE, abolished low reflow at 10 min and increased perfusion at 120 min but did not prevent reperfusion injury at 240 min.Conclusions: PGE(1) and iloprost enhance muscle blood flow at 4-h reperfusion, though neither abolishes low reflow; PGE, improved flow at 10 and 120 min but not after 240 min. This study demonstrates a potentially beneficial role for prostaglandins in improving muscle blood flow in skeletal muscle ischaemia-reperfusion injury.
|Title:||Do prostaglandins have a salutary role in skeletal muscle ischaemia-reperfusion injury?|
|Keywords:||prostaglandin, ischaemia, reperfusion injury, iloprost, skeletal muscle, ENDOTHELIAL-CELLS, BLOOD-FLOW, NEUTROPHIL ACTIVATION, ARACHIDONIC-ACID, CREMASTER MUSCLE, ISCHEMIC-INJURY, PROSTACYCLIN, RAT, INHIBITION, RABBIT|
|UCL classification:||UCL > School of BEAMS
UCL > School of BEAMS > Faculty of Engineering Science
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