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Molecular expression systems for anti-DNA antibodies - 2

Kumar, S; Kalsi, JK; Ravirajan, CT; Latchman, DS; Pearl, LH; Isenberg, DA; (2002) Molecular expression systems for anti-DNA antibodies - 2. LUPUS , 11 (12) 833 - 842. 10.1191/0961203302lu304rr.

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Abstract

Antibodies to double-stranded DNA are the best-known serological markers of systemic lupus erythematosus, and are closely associated with its renal pathogenesis. How these antibodies recognize DNA is not fully understood. An understanding of the relationship between the functional attributes of an antibody with the three-dimensional structure of its antigen-combining site would allow an insight into the rules that dictate auto-antibody-nucleic acid interaction and consequent pathogenicity of the autoantibody. Data from such studies could assist the development of novel drugs as an approach to specific therapies that. can inhibit or disrupt protein-nucleic acid interactions. A full understanding of the binding specificities can be achieved only by experimental determination of detailed three-dimensional structure of these antibodies alone, and of their complexes with specific DNA antigens. A prerequisite of such a study is the ability to produce multimilligram quantities of the antibody protein. However, these antibodies are particularly difficult to express, probably due to their DNA-binding activity. This review attempts to focus on the recent developments on the over-expression of anti-DNA antibody fragments in heterologous cell expression systems and their purification to homogeneity that would in turn allow their structural studies via crystallization.

Type: Article
Title: Molecular expression systems for anti-DNA antibodies - 2
DOI: 10.1191/0961203302lu304rr
Keywords: human anti-DNA antibodies, heterologous cell expression systems, cloning, over-expression, purification, IMMUNOGLOBULIN FAB FRAGMENT, SINGLE-STRANDED-DNA, V-LAMBDA REPERTOIRE, ESCHERICHIA-COLI, HUMAN AUTOANTIBODIES, LUPUS-ERYTHEMATOSUS, BINDING CHARACTERISTICS, CHAIN AUTOANTIBODY, CROSS-REACTIVITY, REGION GENES
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH - Directors Office
URI: http://discovery.ucl.ac.uk/id/eprint/130950
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