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Establishment of defined mixed bacterial plaques on teeth in a laboratory microcosm (artificial mouth)

Donoghue, HD; Perrons, CJ; (1988) Establishment of defined mixed bacterial plaques on teeth in a laboratory microcosm (artificial mouth). Microbial Ecology in Health and Disease , 1 193 - 200. Green open access


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Actinomyces viscosus WVU627, 'Streptococcus mitior' LPA-1 and Veillonella alcalescens OMZ193 were grown in pure or mixed culture on teeth in a model mouth which was supplied with synthetic saliva and an intermittent nutrient supplement containing 1% (w/v) glucose. All organisms became established in pure culture although V. alcalescens colonised very badly unless 1% (w/v) lactate was supplied in place of the glucose. Mixed cultures of all three organisms were readily established. In general, numbers and proportions of species varied widely in mixed culture, but were comparable to those observed in human dental plaque studies. When grown in association with A. viscosus and 'S. mitior', V. alcalescens achieved similar numbers to pure cultures supplied with lactate, thus demonstrating that a food chain existed. Higher viable counts of 'S. mitior' were obtained from mixed plaques, compared with pure cultures. However, less A. viscosus could be isolated when co-cultured with streptococci and veillonellae. Comparison of differential viable counts at 45 h, 66 h and 90 h after inoculation with these three organisms showed that both veillonellae and actinomyces increased in numbers and proportions with incubation time. Streptococcus mutans C67-1 became established when inoculated together with the other organisms, although it attained lower numbers compared with pure cultures. It appeared that S. mutans antagonised A. viscosus and partially displaced this organism in mixed plaques which contained all four species.

Type: Article
Title: Establishment of defined mixed bacterial plaques on teeth in a laboratory microcosm (artificial mouth)
Open access status: An open access version is available from UCL Discovery
Publisher version: http://www.microbecolhealthdis.net/index.php/mehd/...
Language: English
Additional information: Authors contributing to Microbial Ecology in Health & Disease agree to publish their articles under the Creative Commons Attribution-Noncommercial 3.0 Unported license, allowing third parties to share their work (copy, distribute, transmit) and to adapt it, under the condition that the authors are given credit, that the work is not used for commercial purposes, and that in the event of reuse or distribution, the terms of this license are made clear.
Keywords: Actinomyces viscosus, 'Streptococcus mitior', Streptococcus mutans, Veillonella alcalescens, Model mouth, Microbial interactions
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences
URI: http://discovery.ucl.ac.uk/id/eprint/1309103
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