ROBERTS, PJ and PIZZEY, AR and LINCH, DC (1993) THE EFFECT OF INHIBITION OF LEUKOTRIENE SYNTHESIS ON THE ACTIVITY OF INTERLEUKIN-8 AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR. MEDIAT INFLAMM , 2 (3) 211 - 216.
THE cytokines interleukin-8 (IL-8) and granulocyte-macrophage colony-stimulating factor (GM-CSF) enhanced the extracellular release of arachidonate metabolites from ionophore-stimulated neutrophils by 145 +/- 10% (mean +/- S.E.M., n = 13) and 182 +/- 11% (n = 16), respectively. To determine whether enhanced leukotriene production mediates the effects of these cytokines on neutrophil activity, two different specific arachidonate 5-lipoxygenase (5-LO) inhibitors, piriprost and MK-886, were used to inhibit leukotriene synthesis. Neither inhibitor affected the upregulation of CD11b beta2-integrin expression or priming of superoxide generation stimulated by IL-8 and GM-CSF. It is concluded that leukotrienes do not mediate either the direct or priming effects of these cytokines and that these classes of anti-inflammatory drugs are therefore unlikely to inhibit the effects of IL-8 and GM-CSF on neutrophil activation.
|Title:||THE EFFECT OF INHIBITION OF LEUKOTRIENE SYNTHESIS ON THE ACTIVITY OF INTERLEUKIN-8 AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR|
|Open access status:||An open access publication|
|Keywords:||GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR, BETA-2-INTEGRIN, INTERLEUKIN-8, LEUKOTRIENES, 5-LIPOXYGENASE INHIBITORS, NEUTROPHIL, SUPEROXIDE, RECOMBINANT HUMAN GRANULOCYTE, TUMOR-NECROSIS-FACTOR, PLATELET-ACTIVATING-FACTOR, ARACHIDONIC-ACID RELEASE, FC-GAMMA-RII, HUMAN-NEUTROPHILS, POLYMORPHONUCLEAR LEUKOCYTES, EXPRESSION, ADHESION, PATHWAYS|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute > Research Department of Haematology|
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