ROBERTS, PJ; PIZZEY, AR; LINCH, DC; (1993) The effect of inhibition of leukotriene synthesis on the activity of interleukin-8 and granulocyte-macrophage colony-stimulating factor. Mediators of Inflammation , 2 (3) 211- 216. 10.1155/S0962935193000286.
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The cytokines interleukin-8 (IL-8) and granulocyte-macrophage colony-stimulating factor (GM-CSF) enhanced the extracellular release of arachidonate metabolites from ionophore-stimulated neutrophils by 145 +/- 10% (mean +/- S.E.M., n = 13) and 182 +/- 11% (n = 16), respectively. To determine whether enhanced leukotriene production mediates the effects of these cytokines on neutrophil activity, two different specific arachidonate 5-lipoxygenase (5-LO) inhibitors, piriprost and MK-886, were used to inhibit leukotriene synthesis. Neither inhibitor affected the upregulation of CD11b beta2-integrin expression or priming of superoxide generation stimulated by IL-8 and GM-CSF. It is concluded that leukotrienes do not mediate either the direct or priming effects of these cytokines and that these classes of anti-inflammatory drugs are therefore unlikely to inhibit the effects of IL-8 and GM-CSF on neutrophil activation.
|Title:||The effect of inhibition of leukotriene synthesis on the activity of interleukin-8 and granulocyte-macrophage colony-stimulating factor|
|Open access status:||An open access publication. A version is also available from UCL Discovery.|
|Additional information:||This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.|
|Keywords:||granulocyte-macrophage colony-stimulating factor, beta-2-integrin, interleukin-8, leukotrienes, 5-lipoxygenase inhibitors, neutrophil, superoxide, recombinant human granulocyte, tumor-necrosis-factor, platelet-activating-factor, arachidonic-acid release, fc-gamma-rii, human-neutrophils, polymorphonuclear leukocytes, expression, adhesion, pathways|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute > Research Department of Haematology|
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