UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

X-CHROMOSOME INACTIVATION PATTERNS USING HPRT AND PGK POLYMORPHISMS IN HEMATOLOGICALLY NORMAL AND POSTCHEMOTHERAPY FEMALES

GALE, RE; WHEADON, H; LINCH, DC; (1991) X-CHROMOSOME INACTIVATION PATTERNS USING HPRT AND PGK POLYMORPHISMS IN HEMATOLOGICALLY NORMAL AND POSTCHEMOTHERAPY FEMALES. BRIT J HAEMATOL , 79 (2) 193 - 197.

Full text not available from this repository.

Abstract

Clonal analysis using X-chromosome inactivation patterns of the hypoxanthine phosphoribosyl transferase (HPRT) and phosphoglycerate kinase (PGK) genes has been used in the assessment of haematological malignancies. In many analyses normal haemopoietic tissue or constitutive DNA from each individual has not been readily available and it has been assumed that when the ratio of the percentage of the two alleles remaining after differential methylation enzyme digestion is > 3/1 then an oligoclonal/monoclonal population of cells is present. We report here in a study of 65 haematologically normal females informative for HPRT or PGK that 15 (23%) had a skewed Lyonization pattern with clonal analysis ratios > 3/1. No correlation was observed between age and the X-chromosome inactivation pattern to suggest the detection of pre-malignant clonal states. The incidence was similar in 23 females who had received conventional combination chemotherapy, with six women (26%) having a ratio > 3/1. In the majority of individuals therefore, conventional chemotherapy does not appear to alter the patient's X-chromosome inactivation pattern. These results indicate that caution must be exercised in interpreting the results of clonal analysis of any given individual.

Type: Article
Title: X-CHROMOSOME INACTIVATION PATTERNS USING HPRT AND PGK POLYMORPHISMS IN HEMATOLOGICALLY NORMAL AND POSTCHEMOTHERAPY FEMALES
Keywords: CLONAL ANALYSIS, MYELOPROLIFERATIVE DISORDERS, PROBES, DIAGNOSIS, LEUKEMIA
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: http://discovery.ucl.ac.uk/id/eprint/1308275
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item