Lorenz, A; Osman, F; Folkyte, V; Sofueva, S; Whitby, MC; (2009) Fbh1 Limits Rad51-Dependent Recombination at Blocked Replication Forks. MOL CELL BIOL , 29 (17) 4742 - 4756. 10.1128/MCB.00471-09.
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Controlling the loading of Rad51 onto DNA is important for governing when and how homologous recombination is used. Here we use a combination of genetic assays and indirect immunofluorescence to show that the F-box DNA helicase (Fbh1) functions in direct opposition to the Rad52 orthologue Rad22 to curb Rad51 loading onto DNA in fission yeast. Surprisingly, this activity is unnecessary for limiting spontaneous direct-repeat recombination. Instead it appears to play an important role in preventing recombination when replication forks are blocked and/or broken. When overexpressed, Fbh1 specifically reduces replication fork block- induced recombination, as well as the number of Rad51 nuclear foci that are induced by replicative stress. These abilities are dependent on its DNA helicase/translocase activity, suggesting that Fbh1 exerts its control on recombination by acting as a Rad51 disruptase. In accord with this, overexpression of Fbh1 also suppresses the high levels of recombinant formation and Rad51 accumulation at a site-specific replication fork barrier in a strain lacking the Rad51 disruptase Srs2. Similarly overexpression of Srs2 suppresses replication fork block-induced gene conversion events in an fbh1 Delta mutant, although an inability to suppress deletion events suggests that Fbh1 has a distinct functionality, which is not readily substituted by Srs2.
|Title:||Fbh1 Limits Rad51-Dependent Recombination at Blocked Replication Forks|
|Keywords:||BOX DNA HELICASE, CHROMOSOME FRAGILE SITES, STRAND BREAK REPAIR, FISSION YEAST, HOMOLOGOUS RECOMBINATION, SACCHAROMYCES-CEREVISIAE, SCHIZOSACCHAROMYCES-POMBE, F-BOX, ESCHERICHIA-COLI, SRS2 HELICASE|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute > Research Department of Cancer Biology|
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