UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Quantitation of autophagy by luciferase release assay

Ketteler, R; Seed, B; (2008) Quantitation of autophagy by luciferase release assay. AUTOPHAGY , 4 (6) 801 - 806.

Full text not available from this repository.

Abstract

Autophagy is a cellular process that has been defined and analyzed almost entirely by qualitative measures. In no small part, this is attributable to the absence of robust quantitative assays that can easily and reliably permit the progress of key steps in autophagy to be assessed. We have recently developed a cell-based assay that specifically measures proteolytic cleavage of a tripartite sensor protein by the autophagy protease ATG4B. Activation of ATG4B results in release of Gaussia luciferase from cells that can be non-invasively harvested from cellular supernatants. Here, we compare this technique to existing methods and propose that this type of assay will be suitable for genome-wide functional screens and in vivo analysis of autophagy.

Type: Article
Title: Quantitation of autophagy by luciferase release assay
Keywords: autophagy, assay, gaussia, luciferase, secretion, screen, in vivo, ISOLATED RAT HEPATOCYTES, IN-VIVO, MONITORING AUTOPHAGY, PROTEIN-DEGRADATION, REGULATES AUTOPHAGY, MAMMALIAN AUTOPHAGY, GAUSSIA LUCIFERASE, HUMAN ATG4B, LC3, CELLS
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Life Sciences
URI: http://discovery.ucl.ac.uk/id/eprint/1308075
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item