UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Identification of the minimal replicon of the streptomycete plasmid pIJ101.

Zaman, S; Richards, H; Ward, J; (1993) Identification of the minimal replicon of the streptomycete plasmid pIJ101. Plasmid , 29 (1) pp. 57-62. 10.1006/plas.1993.1007.

Full text not available from this repository.

Abstract

The minimal replicon of pIJ101 was defined to a 2.0-kb BalI-SacII fragment containing the rep ORF and a non-coding region of DNA, but not orf56 (pQR435). This non-coding region of DNA was found to be crucial for plasmid replication in Streptomyces lividans and may contain either the origin of replication or the rep promoter (or both). A 1.4-kb NotI-SacII fragment containing only the rep ORF produced a 54-kDa protein in vitro when expressed from the lacZ promoter. pQR435 was structurally unstable in S. lividans and accumulated single-stranded (ss)DNA. The insertion of the site for second-strand initiation (ssi) into pQR435 did not prevent the plasmid from rearranging or from accumulating ssDNA.

Type: Article
Title: Identification of the minimal replicon of the streptomycete plasmid pIJ101.
Location: United States
DOI: 10.1006/plas.1993.1007
Keywords: DNA Replication, DNA, Bacterial, DNA, Single-Stranded, Genes, Bacterial, Open Reading Frames, Plasmids, Replicon, Restriction Mapping, Streptomyces
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: http://discovery.ucl.ac.uk/id/eprint/1307262
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item