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Stability of plasmid vector plJ303 in Streptomyces lividans TK24 during laboratory-scale fermentations.

Wrigley-Jones, CA; Richards, H; Thomas, CR; Ward, J; (1993) Stability of plasmid vector plJ303 in Streptomyces lividans TK24 during laboratory-scale fermentations. Biotechnol Bioeng , 41 (1) pp. 148-155. 10.1002/bit.260410119.

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Abstract

Plasmid plJ303 stability in Streptomyces lividans cultures has been studied by measuring plasmid copy number under various growth conditions. An increase in mean plasmid copy number was normally seen during early rapid growth in both shaken culture and stirred vessel fermentations at 28 degrees C. Maximum copy numbers were consistently attained in early stationary phase followed by a decline (of variable amount) upon further incubation. The imposition of environmental stress (high growth temperature, i.e., 37 degrees C, and low dissolved oxygen tension, i.e., <5% air saturation) led to a plasmid copy number of zero and a 50% reduction, respectively. Interestingly, the relative proportions of plasmid topoisomers changed with time since progressively more supercoiled forms were observed throughout the stationary phase. Plasmid dimers were also observed in some cultures, and no evidence of structural plasmid instability was found. In general, this host-vector system seemed remarkably stable under normal growth conditions. However, copious organic acid production by the host was observed and was thought to be undesirable for good heterologous gene expression of a secreted protein.

Type: Article
Title: Stability of plasmid vector plJ303 in Streptomyces lividans TK24 during laboratory-scale fermentations.
Location: United States
DOI: 10.1002/bit.260410119
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: http://discovery.ucl.ac.uk/id/eprint/1307261
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