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Isolation of developmentally regulated genes by differential display screening of cDNA libraries.

Gupta, R; Thomas, P; Beddington, RS; Rigby, PW; (1998) Isolation of developmentally regulated genes by differential display screening of cDNA libraries. Nucleic Acids Res , 26 (19) pp. 4538-4539. 10.1093/nar/26.19.4538.

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Abstract

mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Using plasmid Southern blot hybridisation as a secondary screen, we are able to identify such genes and show by whole-mount in situ hybridisation that their expression pattern is that expected from the differential display profile.

Type: Article
Title: Isolation of developmentally regulated genes by differential display screening of cDNA libraries.
Location: England
DOI: 10.1093/nar/26.19.4538
Keywords: Animals, DNA, Complementary, Embryonic and Fetal Development, Female, Gene Expression Regulation, Gene Library, Mice, Plasmids, Polymerase Chain Reaction, Pregnancy
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: http://discovery.ucl.ac.uk/id/eprint/1306065
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