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Cut2 proteolysis required for sister-chromatid seperation in fission yeast

Funabiki, H; Yamano, H; Kumada, K; Nagao, K; Hunt, T; Yanagida, M; (1996) Cut2 proteolysis required for sister-chromatid seperation in fission yeast. Nature , 381 (6581) pp. 438-441.

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Abstract

Although mitotic cyclins are well-known substrates for ubiquitin-mediated proteolysis at the metaphase-anaphase transition, their degradation is not essential for separation of sister chromatids; several lines of evidence suggest that proteolysis of other protein(s) is required, however. Here we report the anaphase-specific proteolysis of the Schizosaccharomyces pombe Cut2 protein, which is essential for sister-chromatid separation. Cut2 is located in the nucleus, where it is concentrated along the short metaphase spindle. The rapid degradation of Cut2 at anaphase requires its amino-terminal region and the activity of Cut9 (ref. 14), a component of the 20S cyclosome/anaphase-promoting complex (APC), which is necessary for cyclin destruction. Expression of non-degradable Cut2 blocks sister-chromatid separation but not cell-cycle progression. This defect can be overcome by grafting the N terminus of cyclin B onto the truncated Cut2, demonstrating that the regulated proteolysis of Cut2 is essential for sister-chromatid separation.

Type: Article
Title: Cut2 proteolysis required for sister-chromatid seperation in fission yeast
Publisher version: http://www.ncbi.nlm.nih.gov/pubmed/8632802
Additional information: Funabiki, H Yamano, H Kumada, K Nagao, K Hunt, T Yanagida, M Research Support, Non-U.S. Gov't England Nature Nature. 1996 May 30;381(6581):438-41. Although mitotic cyclins are well-known substrates for ubiquitin-mediated proteolysis at the metaphase-anaphase transition, their degradation is not essential for separation of sister chromatids; several lines of evidence suggest that proteolysis of other protein(s) is required, however. Here we report the anaphase-specific proteolysis of the Schizosaccharomyces pombe Cut2 protein, which is essential for sister-chromatid separation. Cut2 is located in the nucleus, where it is concentrated along the short metaphase spindle. The rapid degradation of Cut2 at anaphase requires its amino-terminal region and the activity of Cut9 (ref. 14), a component of the 20S cyclosome/anaphase-promoting complex (APC), which is necessary for cyclin destruction. Expression of non-degradable Cut2 blocks sister-chromatid separation but not cell-cycle progression. This defect can be overcome by grafting the N terminus of cyclin B onto the truncated Cut2, demonstrating that the regulated proteolysis of Cut2 is essential for sister-chromatid separation.
Keywords: Amino Acid Sequence Cell Division/physiology Cell Nucleus/physiology *Chromatids Fungal Proteins/*metabolism Molecular Sequence Data Recombinant Proteins/metabolism Schizosaccharomyces/cytology/*genetics/metabolism
UCL classification: UCL > School of Life and Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute
UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute > Research Department of Cancer Biology
URI: http://discovery.ucl.ac.uk/id/eprint/1305884
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