Collantes, M; Penuelas, I; Alvarez-Erviti, L; Blesa, J; Marti-Climent, JM; Quincoces, G; ... Richter, JA; + view all Collantes, M; Penuelas, I; Alvarez-Erviti, L; Blesa, J; Marti-Climent, JM; Quincoces, G; Delgado, M; Ecay, M; Martinez, A; Arbizu, J; Rodriguez-Oroze, MC; Obeso, J; Richter, JA; - view fewer (2008) Use of C-11-(+)-alpha-dihydrotetrabenazine for the assessment of dopaminergic innervation in animal models of Parkinson's disease. REV ESP MED NUCL , 27 (2) 103 - 111.
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Aim. This study evaluates the utility of C-11-(+)-alpha-dihydrotetrabenazine (C-11-(+)DTBZ) in the quantification of dopaminergic innervation by positron emission tomography (PET) in rat and monkey, two animal species used as animal models of Parkinson's disease.Material and methods. Healthy control animals (n = 10) and the effect of 6-hydroxidopamine (6-OHDA) neurotoxic were studied in rats. F-18-DOPA PET studies and digital quantitative autoradiography were also carried out. Studies with Macaca fascicularis were performed in control and 1-methyl 4-phenyl 1, 2, 3, 6-tetrahydropyridine (MPTP) treated animals.Results. In both species high quality images were generated in which clear uptake of C-11-(+)DTBZ was found in the striatum. C-11-(+)DTBZ uptake quantification was estimated by creating parametric images and binding potential (BP) calculation. BP in control rats was 1.10 +/- 0. 16 (mean +/- standard deviation [SD], whereas 6-OHDA produced a decrease in the uptake depending on the lesion degree. Images obtained with F-18-DOPA were not adequate for the analysis as they did not discriminate the stratum whereas digital quantitative autoradiography studies confirmed the high affinity of striatum by C-11-(+)DTBZ. In monkeys, final BP values were 1.31 and 1.06 and MPTP treatment reduced uptake by 40%.Conclusions. The quality of PET images and the decrease of uptake in 6-OHDA and MPTP lesions show that C-11-(+)DTBZ is an adequate radiotracer for the study of dopaminergic innervation in these animal models.
|Title:||Use of C-11-(+)-alpha-dihydrotetrabenazine for the assessment of dopaminergic innervation in animal models of Parkinson's disease|
|Keywords:||C-11-(+)-alpha-dihydrotetrabenazine, PET, dopaminergic system, rat, monkey, POSITRON-EMISSION-TOMOGRAPHY, VESICULAR MONOAMINE TRANSPORTER, NERVE-TERMINALS, RODENT MODEL, HUMAN BRAIN, PET, BINDING, MONKEYS, VIVO, AUTORADIOGRAPHY|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Neurology > Clinical Neuroscience|
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