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Platelet membrane CD154 and sCD154 in progressive peripheral arterial disease: A pilot study

Young, RS; Naseem, KM; Pasupathy, S; Ahilathirunayagam, S; Chaparala, RPC; Homer-Vanniasinkam, S; (2007) Platelet membrane CD154 and sCD154 in progressive peripheral arterial disease: A pilot study. ATHEROSCLEROSIS , 190 (2) 452 - 458. 10.1016/j.atherosclerosis.2006.02.038.

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Abstract

Objective: The expression and potential role of platelet membrane CD 154 and sCD 154 in atherosclerosis was investigated in patients with peripheral arterial disease.Methods: This prospective observational study measured the expression of platelet-bound CD154 and soluble CD154 (sCD154) in 39 patients with critical limb ischaemia (CLI, n = 15), stable intermittent claudication (SIC, n = 12) and age-matched controls (AMC, 11 = 12). Basal and aaonist-stimulated CD 154, P-selectin expression and fibrinogen binding was measured by whole blood flow cytometry, while sCD 154 was measured in paired plasma samples by ELISA.Results: Basal expression of CD154 on the platelet surface was enhanced in both groups of patients with peripheral arterial disease. However, the critical limb ischaemics showed the highest level of basal expression 0.7 +/- 0.3 [median +/- IQR] and was significantly increased compared to both stable intermittent claudicants, and age-matched controls (P < 0.001). On agonist stimulation with either ADP or thrombin critical limb ischaemics demonstrated greater platelet reactivity and propensity to express CD154 compared to age-matched controls (P < 0.05). Confirmation of the cellular expression of CID 154 results was obtained by measuring sCD 154 concentrations in autologous plasma samples. Here plasma levels of sCD 154 in critical limb ischaemics were significantly greater than both stable intermittent claudicants and age-matched controls (P < 0.005).Conclusions: Enhanced basal platelet expression and increased propensity to express CD 154 and sCD 154 in critical limb ischaemics compared to both controls and patients with stable intermittent claudication support evidence for the role of CD154 in atherogenesis and suggest a novel function in progressive and acute peripheral arterial disease. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

Type:Article
Title:Platelet membrane CD154 and sCD154 in progressive peripheral arterial disease: A pilot study
DOI:10.1016/j.atherosclerosis.2006.02.038
Keywords:CD154, sCD154, atherosclerosis, platelets, arterial occlusive disease, ACUTE CORONARY SYNDROMES, SOLUBLE CD40 LIGAND, SMOOTH-MUSCLE-CELLS, CIRCULATING PLATELETS, ENDOTHELIAL-CELLS, ACTIVATION, ATHEROSCLEROSIS, CD40-CD40L, RISK, INFLAMMATION
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Surgery and Interventional Science (Division of)

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