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Tomographic imaging of Fluorescence Resonance Energy Transfer in highly light scattering media.
In: Wax, AP and Backman, V, (eds.)
BIOMEDICAL APPLICATIONS OF LIGHT SCATTERING IV.
(pp. ? - ?).
SPIE-INT SOC OPTICAL ENGINEERING
Three-dimensional localization of protein conformation changes in turbid media using Forster Resonance Energy Transfer was investigated by tomographic fluorescence lifetime imaging. We show that Forster Resonance Energy Transfer can be localized by reconstructing the quantum yield and lifetime distribution from time-resolved non-invasive boundary measurements. The approach is demonstrated by imaging a highly scattering cylindrical phantom concealing two thin wells containing cytosol preparations of cells expressing a cytosolic genetically-encoded calcium Resonance Energy Transfer. One of the wells contains calcium chloride solution inducing a protein conformation change accompanied with Forster Resonance Energy Transfer. The resulting fluorescence lifetime distribution, the quantum, efficiency, absorption and scattering coefficients were reconstructed.
|Title:||Tomographic imaging of Fluorescence Resonance Energy Transfer in highly light scattering media|
|Event:||Conference on Biomedical Applications of Light Scattering IV|
|Location:||San Francisco, CA|
|Dates:||2010-01-23 - 2010-01-25|
|Keywords:||Diffuse Optical Tomography, Forster Resonance Energy Transfer, Discontinuous Galerkin Method, OPTICAL TOMOGRAPHY, DATA-ACQUISITION, TURBID MEDIA, ILLUMINATION, PARAMETERS, PROTEIN|
|UCL classification:||UCL > School of BEAMS > Faculty of Engineering Science
UCL > School of BEAMS > Faculty of Engineering Science > Computer Science
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