UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Tomographic imaging of Fluorescence Resonance Energy Transfer in highly light scattering media

Soloviev, VY; McGinty, J; Tahir, KB; Laine, R; Stuckey, DW; Mohan, PS; ... Arridge, SR; + view all (2010) Tomographic imaging of Fluorescence Resonance Energy Transfer in highly light scattering media. In: Wax, AP and Backman, V, (eds.) BIOMEDICAL APPLICATIONS OF LIGHT SCATTERING IV. (pp. ? - ?). SPIE-INT SOC OPTICAL ENGINEERING

Full text not available from this repository.

Abstract

Three-dimensional localization of protein conformation changes in turbid media using Forster Resonance Energy Transfer was investigated by tomographic fluorescence lifetime imaging. We show that Forster Resonance Energy Transfer can be localized by reconstructing the quantum yield and lifetime distribution from time-resolved non-invasive boundary measurements. The approach is demonstrated by imaging a highly scattering cylindrical phantom concealing two thin wells containing cytosol preparations of cells expressing a cytosolic genetically-encoded calcium Resonance Energy Transfer. One of the wells contains calcium chloride solution inducing a protein conformation change accompanied with Forster Resonance Energy Transfer. The resulting fluorescence lifetime distribution, the quantum, efficiency, absorption and scattering coefficients were reconstructed.

Type:Proceedings paper
Title:Tomographic imaging of Fluorescence Resonance Energy Transfer in highly light scattering media
Event:Conference on Biomedical Applications of Light Scattering IV
Location:San Francisco, CA
Dates:2010-01-23 - 2010-01-25
ISBN-13:978-0-8194-7969-3
DOI:10.1117/12.840522
Keywords:Diffuse Optical Tomography, Forster Resonance Energy Transfer, Discontinuous Galerkin Method, OPTICAL TOMOGRAPHY, DATA-ACQUISITION, TURBID MEDIA, ILLUMINATION, PARAMETERS, PROTEIN
UCL classification:UCL > School of BEAMS > Faculty of Engineering Science > Computer Science

Archive Staff Only: edit this record