Kempski, H and Austin, N and Roe, A and Chatters, S and Jayasinghe, SN (2008) Pilot study to investigate the possibility of cytogenetic and physiological changes in bio-electrosprayed human lymphocyte cells. REGEN MED , 3 (3) 343 - 349. 10.2217/17460718.104.22.1683.
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Background: We recently pioneered the ability to directly electrospray and electrospin living cells without compromising their viability. These protocols, now referred to as 'bio-electrosprays' and 'cell electrospinning', are rapidly emerging bio-techniques with a plethora of promising applications within the life sciences, in particular to regenerative and therapeutic medicine. Our studies to date, with both bio-electrosprays and cell electrospinning, have demonstrated that a large population of viable cells exist post-treatment, in comparison to controls over both short and long periods as assessed by flow cytometry. Methods: Post-treated mammalian cells are investigated in comparison to controls (culture and needle controls) at a cytogenetic and physiological level. In particular, the study addresses chromosome integrity following these protocols to assess any protocol-inflicted aberrations. Results: The procedures explored failed to inflict any process-driven gross chromosomal aberrations post-treatment. Conclusions: Our preliminary investigations demonstrate no significant compromising affects on the cell's structure at a cytogenetic or physiological level, post-treatment. Thus, further establishing these protocols as unique direct cell-engineering approaches with a host of biological and medical applications, from the development of tissues to perhaps even organs in the future.
|Title:||Pilot study to investigate the possibility of cytogenetic and physiological changes in bio-electrosprayed human lymphocyte cells|
|Keywords:||aerodynamically assisted bio-jetting/threading, bio-electrosprays, cell electiospinning, human blood cells, karyotyping, PRIMARY LIVING ORGANISMS, ELECTRIC-FIELD, METHODOLOGY, JETS|
|UCL classification:||UCL > School of BEAMS > Faculty of Engineering Science > Mechanical Engineering|
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