Miller, OJ; Dalby, PA; (2004) Exposing relationships using directed evolution. TRENDS BIOTECHNOL , 22 (5) 203 - 205. 10.1016/j.tibtech.2004.03.003.
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Functionally related protein structures that have undergone significant mutagenesis and re-arrangement over a large evolutionary time-scale might no longer share enough sequence or structural similarity to be revealed by even the most advanced database searches. Recently, Christ and Winter used directed evolution to obtain functional variants of the RNA-hairpin-binding protein Rop. Using the functional sequences obtained, a structural database search revealed previously unknown similarity to the tRNA-binding region of valy- tRNA synthetase.
|Title:||Exposing relationships using directed evolution|
|Keywords:||IN-VITRO EVOLUTION, PHAGE DISPLAY, BETA-GLUCURONIDASE, BINDING-PROTEINS, ENZYME, AFFINITY, RECOGNITION, SPECIFICITY, STABILITY, LIBRARY|
|UCL classification:||UCL > School of BEAMS > Faculty of Engineering Science > Biochemical Engineering|
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