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INDUCIBLE OPERATION OF THE ERYTHROPOIETIN-3' ENHANCER IN MULTIPLE CELL-LINES - EVIDENCE FOR A WIDESPREAD OXYGEN-SENSING MECHANISM

MAXWELL, PH; PUGH, CW; RATCLIFFE, PJ; (1993) INDUCIBLE OPERATION OF THE ERYTHROPOIETIN-3' ENHANCER IN MULTIPLE CELL-LINES - EVIDENCE FOR A WIDESPREAD OXYGEN-SENSING MECHANISM. P NATL ACAD SCI USA , 90 (6) 2423 - 2427.

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Abstract

Adaptive responses to hypoxia occur in many biological systems. A well-characterized example is the hypoxic induction of the synthesis of erythropoietin, a hormone which regulates erythropoiesis and hence blood oxygen content. The restricted expression of the erythropoietin gene in subsets of cells within kidney and liver has suggested that this specific oxygen-sensing mechanism is restricted to specialized cells in those organs. Using transient transfection of reporter genes coupled to a transcriptional enhancer lying 3' to the erythropoietin gene, we show that an oxygen-sensing system similar, or identical, to that controlling erythropoietin expression is wide-spread in mammalian cells. The extensive distribution of this sensing mechanism contrasts with the restricted expression of erythropoietin, suggesting that it mediates other adaptive responses to hypoxia.

Type:Article
Title:INDUCIBLE OPERATION OF THE ERYTHROPOIETIN-3' ENHANCER IN MULTIPLE CELL-LINES - EVIDENCE FOR A WIDESPREAD OXYGEN-SENSING MECHANISM
Keywords:INSITU HYBRIDIZATION, TRANSGENIC MICE, GENE, EXPRESSION, HYPOXIA, TRANSCRIPTION, LOCALIZATION, PROTEIN, ELEMENT
UCL classification:UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Medicine (Division of)

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