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CELLULAR MECHANISMS OF GRAFT-VERSUS-HOST DISEASE IN A MOUSE MODEL

DEGIORGI, L; LOWDELL, MW; MATOSSIANROGERS, A; (1991) CELLULAR MECHANISMS OF GRAFT-VERSUS-HOST DISEASE IN A MOUSE MODEL. SCAND J IMMUNOL , 33 (5) 567 - 574.

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Abstract

Female CBA/H (H-2k, Mls(b)) mice alloimmunized prior to and during syngeneic pregnancy with DBA/2 (H-2d, Mls(a)) splenocytes gave rise to offspring which resisted graft-versus-host disease (GVHD) following neonatal intraperitoneal inoculation of high doses of DBA/2 spleen cells. Lymphocytes from GVHD-resistant mice tested after 6 weeks of age were unresponsive to DBA/2 stimulator cells in 72 h mixed lymphocyte cultures. Isotope uptake measured 24 h after culture, however, indicated that a considerable early response was made to DBA/2 which later declined. Proliferative responses to BALB/c were also depressed but no early response to this strain was detected. FACS analysis of T-lymphocyte profiles of the GVHD-resistant CBA/H mice revealed a 100% increase in the Lyt-2+ subpopulation compared to normal CBA/H mice. Significant increases in Lyt-2+ cells were also noted in in vito cultures of CBA/H lymphocytes responding to GVHD-resistant CBA/H stimulators. Lymphocytes from GVHD-resistant mice suppressed the proliferative responses of normal CBA/H lymphocytes to alloantigenic but not mitogenic stimulation. Suppression of alloantigenic responses were shown to be specific to DBA/2 and did not affect the response to BALB/c stimulator cells, indicating that both anergy and specific suppressor cells were operative in inducing unresponsiveness.

Type: Article
Title: CELLULAR MECHANISMS OF GRAFT-VERSUS-HOST DISEASE IN A MOUSE MODEL
Keywords: CLASS-I ANTIGENS, SUPPRESSOR CELLS, LYMPHOCYTE-T, TRANSPLANTATION TOLERANCE, NON-H-2 ANTIGENS, SELF-TOLERANCE, SPLEEN-CELLS, VETO CELLS, MICE, MAINTENANCE
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
URI: http://discovery.ucl.ac.uk/id/eprint/128117
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