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A Comparison of Labeling and Label-Free Mass Spectrometry-Based Proteomics Approaches

Patel, VJ; Thalassinos, K; Slade, SE; Connolly, JB; Crombie, A; Murrell, JC; Scrivens, JH; (2009) A Comparison of Labeling and Label-Free Mass Spectrometry-Based Proteomics Approaches. J PROTEOME RES , 8 (7) 3752 - 3759. 10.1021/pr900080y.

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Abstract

The proteome of the recently discovered bacterium Methyiocella silvestris has been characterized using three profiling and comparative proteomics approaches. The organism has been grown on two different substrates enabling variations in protein expression to be identified. The results obtained using the experimental approaches have been compared with respect to number of proteins identified, confidence in identification, sequence coverage and agreement of regulated proteins. The sample preparation, instrumental time and sample loading requirements of the differing experiments are compared and discussed. A preliminary screen of the protein regulation results for biological significance has also been performed.

Type: Article
Title: A Comparison of Labeling and Label-Free Mass Spectrometry-Based Proteomics Approaches
DOI: 10.1021/pr900080y
Keywords: proteomics, quantification, iTRAQ, label-free, methanotroph, mass spectrometry, COMPLEX PROTEIN MIXTURES, LIQUID-CHROMATOGRAPHY, GEL-ELECTROPHORESIS, ABSOLUTE QUANTIFICATION, QUANTITATIVE-ANALYSIS, SHOTGUN PROTEOMICS, CELL-CULTURE, SERUM, TAGS, MS
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: http://discovery.ucl.ac.uk/id/eprint/127861
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