UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

INTRACELLULAR ION IMAGING USING FLUORESCENT DYES - ARTIFACTS AND LIMITS TO RESOLUTION

SILVER, RA; WHITAKER, M; BOLSOVER, SR; (1992) INTRACELLULAR ION IMAGING USING FLUORESCENT DYES - ARTIFACTS AND LIMITS TO RESOLUTION. PFLUG ARCH EUR J PHY , 420 (5-6) 595 - 602.

Full text not available from this repository.

Abstract

Development of highly efficient fluorescent ratio indicators has made imaging of ion concentrations within individual cells possible (Grynkiewicz et al. 1985; Tsien and Poenie 1986). lon imaging is a complex technique and is therefore prone to artefacts. In this paper we investigate the limits of the technique and its potential pitfalls. The spatial resolution of an imaging system is determined for different cell geometries. We describe a technique to increase the time resolution of existing systems by using a single excitation wavelength to measure changes in ion concentration. We demonstrate examples of potential artefacts arising from hardware limitations, image processing and fundamental optics. Methods for recognition and minimization of these problems are discussed.

Type: Article
Title: INTRACELLULAR ION IMAGING USING FLUORESCENT DYES - ARTIFACTS AND LIMITS TO RESOLUTION
Keywords: CALCIUM, IMAGING, MEASUREMENT, ARTIFACT, FLUORESCENCE MICROSCOPE, RESOLUTION, FURA-2, CYTOSOLIC FREE CALCIUM, SPATIAL GRADIENTS, GROWTH CONES, CELLS, CA-2+, FURA-2, NEUTROPHILS, TRANSIENTS, CHANNELS, SINGLE
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/124503
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item