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INTRACELLULAR CYCLIC-AMP PRODUCES EFFECTS OPPOSITE TO THOSE OF CYCLIC-GMP AND CALCIUM ON SHAPE AND MOTILITY OF NEUROBLASTOMA-CELLS

BOLSOVER, SR; GILBERT, SH; SPECTOR, I; (1992) INTRACELLULAR CYCLIC-AMP PRODUCES EFFECTS OPPOSITE TO THOSE OF CYCLIC-GMP AND CALCIUM ON SHAPE AND MOTILITY OF NEUROBLASTOMA-CELLS. CELL MOTIL CYTOSKEL , 22 (2) 99 - 116.

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Abstract

We have directly evaluated the effects of various intracellular second messengers including cyclic nucleotides, calcium ion, and inositol polyphosphates on shape and motility of differentiating mouse neuroblastoma cells. The messengers were microinjected into cells and the responses of the soma, neurite, and growth cone were monitored using time-lapse video microscopy. Each messenger altered cell shape and motility in a characteristic manner. Cyclic AMP promoted lamellipodial expansion, neurite outgrowth, and motility. The other injected messengers opposed motility. Cyclic GMP caused motile structures to freeze and to retract permanently, while the inhibitory effects of calcium injection were concentration-dependent. Small calcium injections affected specifically actin-containing motile structures which froze and retracted temporarily. Intermediate calcium injections caused a strong contraction at the site of injection in all cells. With large injections, cells retracted long neurites, rounded up, and frequently began vigorous blebbing that continued to cell death. Injections of the inositol polyphosphates IP3(1,4,5) and IP4(1,4,5,6) mimicked the effects of small calcium injections, as did electrical stimulation that elicited action potentials. The results suggest that in mouse neuroblastoma cells, intracellular cAMP elevation increases cytoskeletal organization and promotes neurite extension perhaps through an enhancement of cell-substratum adhesion. On the other hand, a rise of intracellular cGMP or intracellular calcium interferes directly with the function and organization of the actin-microfilament system. The integrated action of these second messenger systems may, therefore, operate in vivo to allow substances released from neighboring cells to regulate neuronal architecture.

Type: Article
Title: INTRACELLULAR CYCLIC-AMP PRODUCES EFFECTS OPPOSITE TO THOSE OF CYCLIC-GMP AND CALCIUM ON SHAPE AND MOTILITY OF NEUROBLASTOMA-CELLS
Keywords: MICROINJECTION, 2ND MESSENGERS, CYTOSKELETON, NERVE GROWTH-FACTOR, MICROTUBULE-ASSOCIATED PROTEINS, CELLULAR SIGNAL TRANSDUCTION, MOUSE 3T3 CELLS, NEURITE ELONGATION, INOSITOL 1,4,5-TRISPHOSPHATE, ACTIN-FILAMENTS, CONE MOTILITY, NEURONS, OUTGROWTH
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: http://discovery.ucl.ac.uk/id/eprint/124491
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