Volpi, EV and Chevret, E and Jones, T and Vatcheva, R and Williamson, J and Beck, S and Campbell, RD and Goldsworthy, M and Powis, SH and Ragoussis, J and Trowsdale, J and Sheer, D (2000) Large-scale chromatin organization of the major histocompatibility complex and other regions of human chromosome 6 and its response to interferon in interphase nuclei. J CELL SCI , 113 (9) 1565 - 1576.
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The large-scale chromatin organization of the major histocompatibility complex and other regions of chromosome 6 was studied by three-dimensional image analysis in human cell types with major differences in transcriptional activity. Entire gene clusters were visualized by fluorescence in situ hybridization with multiple locus-specific probes. Individual genomic regions showed distinct configurations in relation to the chromosome 6 terrritory. Large chromatin loops containing several megabases of DNA were observed extending outwards from the surface of the domain defined by the specific chromosome 6 paint. The frequency with which a genomic region was observed on an external chromatin loop was cell type dependent and appeared to be related to the number of active genes in that region. Transcriptional up-regulation of genes in the major histocompatibility complex by interferon-gamma led to an increase in the frequency with which this large gene cluster was found on an external chromatin loop. Our data are consistent with an association between large-scale chromatin organization of specific genomic regions and their transcriptional status.
|Title:||Large-scale chromatin organization of the major histocompatibility complex and other regions of human chromosome 6 and its response to interferon in interphase nuclei|
|Keywords:||human chromosome 6, interphase chromosome, nuclear architecture, MHC, IFN-gamma, CLASS-II GENES, FUNCTIONAL COMPARTMENTALIZATION, MESSENGER-RNA, CELL-CYCLE, MHC GENES, IN-VIVO, EXPRESSION, TRANSCRIPTION, TERRITORIES, ACTIVATION|
|UCL classification:||UCL > School of Life and Medical Sciences > Faculty of Medical Sciences > Wolfson Institute and Cancer Institute Administration > Cancer Institute > Research Department of Cancer Biology|
UCL > School of BEAMS > Faculty of Maths and Physical Sciences > Physics and Astronomy
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