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Three-dimensional in vivo fluorescence diffuse optical tomography of breast cancer in humans

Corlu, A; Choe, R; Durduran, T; Rosen, MA; Schweiger, M; Arridge, SR; ... Yodh, AG; + view all (2007) Three-dimensional in vivo fluorescence diffuse optical tomography of breast cancer in humans. OPT EXPRESS , 15 (11) 6696 - 6716. Gold open access

Abstract

We present three-dimensional (3D) in vivo images of human breast cancer based on fluorescence diffuse optical tomography (FDOT). To our knowledge, this work represents the first reported 3D fluorescence tomography of human breast cancer in vivo. In our protocol, the fluorophore Indocyanine Green (ICG) is injected intravenously. Fluorescence excitation and detection are accomplished in the soft-compression, parallel-plane, transmission geometry using laser sources at 786 nm and spectrally filtered CCD detection. Phantom and in vivo studies confirm the signals are due to ICG fluorescence, rather than tissue autofluorescence and excitation light leakage. Fluorescence images of breast tumors were in good agreement with those of MRI, and with DOT based on endogenous contrast. Tumor-to-normal tissue contrast based on ICG fluorescence was two-to-four-fold higher than contrast based on hemoglobin and scattering parameters. In total the measurements demonstrate that FDOT of breast cancer is feasible and promising. (C) 2007 Optical Society of America.

Type:Article
Title:Three-dimensional in vivo fluorescence diffuse optical tomography of breast cancer in humans
Open access status:An open access publication
Publisher version:http://www.opticsexpress.org
Keywords:SPATIALLY-RESOLVED FLUORESCENCE, NEAR-INFRARED TOMOGRAPHY, TURBID MEDIA, IMAGE-RECONSTRUCTION, CONTRAST AGENTS, FLUOROPHORE CONCENTRATIONS, WAVELENGTH OPTIMIZATION, PHOTODYNAMIC-THERAPY, INDOCYANINE GREEN, SCATTERING MEDIA
UCL classification:UCL > School of BEAMS > Faculty of Engineering Science > Computer Science

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