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C-terminal interactions modulate the affinity of GLAST glutamate transporters in salamander retinal glial cells

Marie, H; Attwell, D; (1999) C-terminal interactions modulate the affinity of GLAST glutamate transporters in salamander retinal glial cells. J PHYSIOL-LONDON , 520 (2) 393 - 397.

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Abstract

1. Proteins that interact with the intracellular carboxy termini of neurotransmitter- and voltage-gated ion channels are known to control the subcellular localization of the channels, localize other proteins near those channels, and modulate channel activity. By contrast, little is known about the control of neurotransmitter transporter function by interacting proteins.2. To competitively disrupt interactions of the C- and N-termini of the GLAST glutamate transporter with other proteins, we dialysed whole-cell patch-clamped retinal glia with peptides identical to the eight amino acids at the C- or N-termini of the transporter, and compared the effect on transporter-mediated currents with dialysis of scrambled versions of the same peptides.3. Dialysis with the N-terminus peptide had no effect on the maximum glutamate-evoked current nor on the glutamate affinity of the transporter. Dialysis with the C-terminus peptide had no effect on the maximum current, but increased the affinity of the transporter for glutamate (compared with scrambled C-terminus peptide, and with N- and scrambled N-terminus peptides: K-m decreased from 16 to 11 mu m)).4.These data suggest that disruption of an interaction between an intracellular protein and the last eight amino acids of the GLAST C-terminus, which have some similarity to the PDZ binding domain of ion channel C-termini, increases the glutamate affinity of GLAST. Thus, the interacting protein decreases the affinity of GLAST transporters.5. Removing the GLAST C-terminus interaction increases the transporter current by 40 % at low glutamate concentrations. Thus, this interaction may significantly slow the removal of low concentrations of glutamate from the extracellular space, and affect the kinetics of retinal cell light responses.

Type: Article
Title: C-terminal interactions modulate the affinity of GLAST glutamate transporters in salamander retinal glial cells
Keywords: DENSITY PROTEIN PSD-95, POTASSIUM CHANNEL, CONDUCTANCE, RECEPTORS, IDENTIFICATION, MEMBRANE, CARRIER, DOMAINS, BINDS, GLT-1
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/122700
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