UCL logo

UCL Discovery

UCL home » Library Services » Electronic resources » UCL Discovery

Subunit-specific association of protein kinase C and the receptor for activated C kinase with GABA type A receptors

Brandon, NJ; Uren, JM; Kittler, JT; Wang, HB; Olsen, R; Parker, PJ; Moss, SJ; (1999) Subunit-specific association of protein kinase C and the receptor for activated C kinase with GABA type A receptors. J NEUROSCI , 19 (21) 9228 - 9234.

Full text not available from this repository.

Abstract

GABA receptors (GABA(A)) are the major sites of fast synaptic inhibition in the brain and can be assembled from five subunit classes: alpha, beta, gamma, delta, and epsilon. Receptor function can be regulated by direct phosphorylation of beta and gamma 2 subunits, but how kinases are targeted to GABA(A) receptors is unknown. Here we show that protein kinase C-beta II (PKC-beta II) is capable of directly binding to the intracellular domain of the receptor beta 1 and beta 3 subunits, but not to those of the alpha 1 or gamma 2 subunits. Moreover, associating PKC-beta II is capable of specifically phosphorylating serine 409 in beta 1 subunit and serines 408/409 within the beta 3 subunit, key residues for modulating GABA(A) receptor function. The receptor for activated C kinase (RACK-1) was found also to bind to the beta 1 subunit intracellular domain, but PKC binding appeared to be independent of this protein. Using immunoprecipitation, the association of PKC isoforms and RACK-1 with neuronal GABA(A) receptors was seen. Furthermore, PKC isoforms associating with neuronal receptors were capable of phosphorylating the receptor beta 3 subunit.Together, these observations suggest GABA(A) receptors are intimately associated with PKC isoforms via a direct interaction with receptor b subunits. This interaction may serve to localize PKC activity to GABA(A) receptors in neurons allowing the rapid regulation of receptor activity by cell-signaling pathways that modify PKC activity.

Type: Article
Title: Subunit-specific association of protein kinase C and the receptor for activated C kinase with GABA type A receptors
Keywords: GABA(A) receptor, beta subunit, PKC, RACK-1, intracellular domain, protein kinase C, A RECEPTOR, BETA-SUBUNIT, PHOSPHORYLATION SITES, INTRACELLULAR DOMAINS, 2 FORMS, CURRENTS, POLYPEPTIDES, SELECTIVITY, EXPRESSION, MODULATION
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: http://discovery.ucl.ac.uk/id/eprint/122671
Downloads since deposit
0Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item